Nature Immunology
3, 975 - 983 (2002)
Published online: 23 September 2002; | doi:10.1038/ni841
Diversity of receptors binding HIV on dendritic cell subsetsStuart G. Turville1, Paul U. Cameron2, Amanda Handley2, George Lin3, Stefan Pöhlmann4, Robert W. Doms4
& Anthony L. Cunningham11
Center for Virus Research, Westmead Millennium Institute, Westmead Hospital and University of Sydney, Sydney, NSW 2145 and Australian National Centre for HIV Virology Research, Australia. 2
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052. 3
Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. 4
Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Correspondence should be addressed to Anthony L. Cunningham Tony_cunningham@wmi.usyd.edu.auThe ability of HIV-1 to use dendritic cells (DCs) for transport and to transfer virus to activated T cells in the lymph node may be crucial in early HIV-1 pathogenesis. We have characterized primary DCs for the receptors involved in viral envelope attachment and observed that C-type lectin receptor (CLR) binding was predominant in skin DCs, whereas binding to emigrating and tonsil DCs was CD4-dependent. No one CLR was solely responsible for envelope binding on all skin DC subsets. DC-SIGN (DC-specific ICAM-3−grabbing nonintegrin) was only expressed by CD14+CDlalo dermal DCs. The mannose receptor was expressed by CD1ahi and CD14+CDlalo dermal DCs, and langerin was expressed by Langerhans cells. The diversity of CLRs able to bind HIV-1 in skin DCs may reflect their ability to bind a range of microbial glycoproteins.
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