Crucial importance of PKC-(I) in LFA-1−mediated locomotion of activated T cells
Y. Volkov1, 2, A. Long3, S. McGrath2, D. Ni Eidhin2
& D. Kelleher1, 2
1
Dublin Molecular Medicine Centre, Trinity College, Dublin 8, Ireland.
2
Department of Clinical Medicine, Trinity College, Dublin 8, Ireland.
3
Department of Biochemistry, Royal College of Surgeons, St Stephen's Green, Dublin 2, Ireland.
Correspondence should be addressed to Y. Volkov yvolkov@tcd.ie
Crawling T cell locomotion in which activated lymphocyte function−associated antigen 1 (LFA-1) integrins participate is associated with translocation of the protein kinase C- (PKC-) isoenzyme to the microtubule cytoskeleton. In normal T cells and T lymphoma cell lines, this type of motility is accompanied by PKC-−sensitive cytoskeletal rearrangements and the formation of trailing cell extensions, which are supported by microtubules. Expression of PKC-(I) and enhanced green fluorescent protein (EGFP) in nonmotile PKC-−deficient T cells restored their locomotory behavior in response to a triggering stimulus delivered via LFA-1 and correlated directly with the degree of cell polarization. We have also shown that PKC-(I) is a component of the tubulin-enriched LFA-1−cytoskeletal complex assembled upon LFA-1 cross-linking. These observations may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation.
(I) in LFA-1−mediated locomotion of activated T cells
(PKC-
