Nature Immunology
2, 338 - 345 (2001)
doi:10.1038/86342
A CD14-independent LPS receptor clusterKathy Triantafilou1, 3, Martha Triantafilou1
& Russell L. Dedrick21
University of Essex, Department of Biological Sciences, Central Campus, Wivenhoe Park, Colchester CO4 3SQ, UK. 2
Molecular Immunology, XOMA (US) LLC, 2910 Seventh Street, Berkeley, CA 94710, UK. 3
Present address: University of Portsmouth, School of Biological Sciences, King Henry Building, King Henry I Street, Portsmouth PO1 2DY, UK.
Correspondence should be addressed to Kathy Triantafilou kathy.triantafilou@port.ac.ukBacterial lipopolysaccharide (LPS), the major structural component of the outer wall of Gram-negative bacteria, is a potent initiator of an inflammatory response and serves as an indicator of bacterial infection. Although CD14 has been identified as the main LPS receptor, accumulating evidence has suggested the possible existence of other functional receptor(s). In this study, using affinity chromatography, peptide mass fingerprinting and fluorescence resonance energy transfer, we have identified four new proteins that form an activation cluster after LPS ligation and are involved in LPS signal transduction. Here we present evidence that implicates heat shock proteins 70 and 90, chemokine receptor 4 and growth differentiation factor 5 as the main mediators of activation by bacterial lipopolysaccharide.
|
