Nature Immunology
1, 502 - 509 (2000)
doi:10.1038/82751
OmpA targets dendritic cells, induces their maturation and delivers antigen into the MHC class I presentation pathwayPascale Jeannin1, Toufic Renno1, 3, Liliane Goetsch1, 3, Isabelle Miconnet2, 3, Jean-Pierre Aubry1, 3, Yves Delneste1, Nathalie Herbault1, Thierry Baussant1, Giovanni Magistrelli1, Caroline Soulas1, Pedro Romero2, Jean-Charles Cerottini2
& Jean-Yves Bonnefoy11
Centre d'Immunologie Pierre Fabre, 5, Avenue Napoléon III, F-74164 Saint-Julien en Genevois, France. 2
Ludwig Institute for Cancer research, Lausanne Branch, University of Lausanne, CH-1406 Epalinges, Switzerland. 3
These authors contributed equally to this work.
Correspondence should be addressed to Pascale Jeannin pascale.jeannin@pierre-fabre.comWe analyzed the interaction between a bacterial cell wall protein and dendritic cells (DCs). Outer membrane protein A from Klebsiella pneumoniae (kpOmpA) specifically bound to professional antigen presenting cells and was endocytosed by immature DCs via a receptor-dependent mechanism. kpOmpA signaled through Toll-like receptor 2, induced DCs to produce interleukin 12 and induced maturation of DCs. Whole antigen that was coupled to kpOmpA and injected into mice was taken up by DCs and delivered to the conventional cytosolic MHC class I presentation pathway. kpOmpA also primed antigen-specific CD8+ CTLs in the absence of CD4+ T cell help or adjuvant and elicited therapeutic immunity to antigen-expressing tumors. Thus, OmpA belongs to a class of proteins that are able to elicit CTL responses to exogenous antigen.
|
