Nature Immunology
1, 311 - 316 (2000)
doi:10.1038/79758
Kinetics of dendritic cell activation: impact on priming of TH1,
TH2 and nonpolarized T cellsAnja Langenkamp1, 2, Mara Messi1, Antonio Lanzavecchia1
& Federica Sallusto1, 21
Institute for Research in Biomedicine,
Via Vincenzo Vela 6, CH-6500 Bellinzona,
Switzerland. 2
Basel Institute for Immunology, Grenzacherstrasse
487, CH-4005 Basel, Switzerland.
Correspondence should be addressed to Federica Sallusto federica.sallusto@irb.unisi.chTo prime immune responses, dendritic cells (DCs) need to be activated to
acquire T cell stimulatory capacity. Although some stimuli trigger interleukin
12 (IL-12) production that leads to T helper cell type 1 (TH1)
polarization, others fail to do so and favor TH2 polarization.
We show that after activation by lipopolysaccharide, DCs produced IL-12 only
transiently and became refractory to further stimulation. The exhaustion of
cytokine production impacted the T cell polarizing process. Soon after stimulation
DCs primed strong TH1 responses, whereas at later time points the
same cells preferentially primed TH2 and nonpolarized T cells.
These findings indicate that during an immune response, T cell priming conditions
may change in the lymph nodes, suggesting another mechanism for the regulation
of effector and memory T cells.
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