Nature Immunology
1, 113 - 118 (2000)
doi:10.1038/77783
Secretion of microbicidal  -defensins by intestinal Paneth cells
in response to bacteriaTokiyoshi Ayabe1, Donald P. Satchell1, Carole L. Wilson3, William C. Parks3, 4, Michael E. Selsted1, 2
& Andre J. Ouellette1, 21
Department of Pathology, College of Medicine, University
of California, Irvine, CA 92697,
USA. 2
Department of Microbiology and Molecular Genetics,
College of Medicine, University of California, Irvine,
CA 92697, USA. 3
Department of Pediatrics, Washington University School
of Medicine, St. Louis, MO 63110,
USA. 4
Department of Cell Biology & Physiology, Washington
University School of Medicine, St. Louis, MO
63110, USA.
Correspondence should be addressed to Andre J. Ouellette aouellet@uci.eduPaneth cells in mouse small intestinal crypts secrete granules rich in
microbicidal peptides when exposed to bacteria or bacterial antigens. The
dose-dependent secretion occurs within minutes and  -defensins, or cryptdins,
account for 70% of the released bactericidal peptide activity. Gram-negative
bacteria, Gram-positive bacteria, lipopolysaccharide, lipoteichoic acid, lipid
A and muramyl dipeptide elicit cryptdin secretion. Live fungi and protozoa,
however, do not stimulate degranulation. Thus intestinal Paneth cells contribute
to innate immunity by sensing bacteria and bacterial antigens, and discharge
microbicidal peptides at effective concentrations accordingly.
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