Nature Genetics
8, 70 - 76 (1994)
doi:10.1038/ng0994-70
A radiation hybrid map of 506 STS markers spanning human chromosome 11M.R. James1, 2, 11, 12, C.W. Richard III3, J.-J. Schott1, 2, C. Yousry1, 2, K. Clark4, J. Bell4, J.D. Terwilliger5, J. Kazan6, C. Dubay1, 2, A. Vignal7, M. Agrapart1, 2, T. Imai8, Y. Nakamura8, M. Polymeropoulos9, J. Weissenbach6, 7, D.R. Cox10
& G.M. Lathrop1, 2
1INSERM U358
2Centre d'Etude du Polymorphisme Humain, 27 rue Juliette Dodu, Paris, France
3Departments of Psychiatry and Human Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
4Institute of Molecular Medicine, John Radcttffe Hospital, Oxford OX39DU, UK
5Department of Genetics and Development, Columbia University, New York New York 10032, USA
6CNRS URA 1445, Institut Pasteur, Paris, France
7Généthon, 1 rue de l'Intemationale, 91000 Evry, France
8Department of Biochemistry, Cancer Institute, Kami-Ikebukuro, Tokyo 170, Japan
9National Center for Human Genome Research, National Institutes of Health, Building 49, Bethesda, Maryland 20892, USA 10
10Department of Genetics, Stanford University, Stanford, California 94305, USA
11Present address: The Wellcome Trust Centre for Human Genetics, Nuffield Orthopaedic Centre, Windmill Road, Headington, Oxford, OX3 7LD, UK
12Correspondence should be addressed to M.R.J. We present a high resolution radiation hybrid map of human chromosome 11 using 506 sequence tagged sites (STSs) scored on a panel of 86 radiation hybrids. The 506 STSs fall into 299 unique positions (average resolution of about 480 kilobases (kb)) that span the whole chromosome. A subset of 260 STSs (143 positions) form a framework map that has a resolution of approximately 1 megabase between adjacent positions and is ordered with odds of at least 1,000:1. The centromere was clearly defined with pericentric markers unambiguously assigned to the short or long arm. The map contains most genes (125) and expressed sequence tags (26) currently assigned to chromosome 11 and more than half of the STSs are polymorphic microsatellite loci. These markers and the map can be used for high resolution physical and genetic mapping.
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