Nature Genetics
6, 33 - 40 (1994)
doi:10.1038/ng0194-33
Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS methodY. Hayashizaki1, 2, H. Shibata1, 2, S. Hirotsune1, 2, H. Sugino1, Y. Okazaki1, 2, N. Sasaki1, K. Hirose1, H. Imoto1, H. Okuizumi1, M. Muramatsu1, H. Komatsubara2, T. Shiroishi3, K. Moriwaki3, M. Katsuki4, N. Hatano5, H. Sasaki5, T. Ueda6, N. Mise7, N. Takagi7, C. Plass8
& V.M. Chapman8
1Gene Bank, RIKEN Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305 Japan
2Department of Bioscience, National Cardiovascular Center, Research Institute, 5-7-1 Fujishiro-dai Suita, Osaka, 565 Japan
3Department of Cell Genetics, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuokaken, 411 Japan
4Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, 812, Japan
5Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, 812, Japan
6Research Laboratory for Molecular Genetics, Niigata University, 757, Ichiban-chou, Asahimachidoori, Niigata, 951, Japan
7Division of Biological Science Graduate School of Environmental Earth Science, Hokkaido University, North 10 West 8, Kitaku, Sapporo, 060, Japan
8Molecular and Cellular Biology Department, Roswell Park Cancer Institute, Elm and Carton Streets, Buffalo, New York 14263, USA A new imprinted gene has been discovered in mice using the technique of restriction landmark genomic scanning (RGLS) with methylation sensitive enzymes. Eight out of 3,100 strain−specific Notl and BssHII spots were identified as imprinted in reciprocal F1 hybrids. Subsequently, we isolated a genomic clone for one locus on proximal chromosome 11 near the Glns locus, an imprinted region in uniparental disomic mice, and its corresponding cDNA clone. Expression of this transcript from the paternal allele was established using RT−PCR of reciprocal F1−hybrid mice. The amino−acid sequence deduced from the cDNA showed significant homology to the U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit.
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