Nature Genetics
5, 259 - 265 (1993)
doi:10.1038/ng1193-259
Widespread expression of the human and rat Huntington's disease gene in brain and nonneural tissuesTheresa V. Strong1, 2, Danilo A. Tagle1, 2, John M. Valdes3, Lawrence W. Elmer1, 4, Karina Boehm1, Manju Swaroop1, Kevin W. Kaatz4, Francis S. Collins1, 2, 5
& Roger L. Albin4
1Division of Molecular Medicine and Genetics, Department of Internal Medicine, 4570 MSRB II
2Department of Human Genetics, University of Michigan, Ann Arbor, Michigan 48109, USA
3Cell and Molecular Biology Program, University of Michigan, Ann Arbor, Michigan 48109, USA
4Department of Neurology, Neuroscience Laboratory Building, University of Michigan, 1103 E. Huron, Ann Arbor, Michigan 48104, USA
5National Center for Human Genome Research, NIH, Bethesda, MD 20892, USA Correspondence should be addressed to F.C. We have used RNA in situ hybridization to study the regional expression of the Huntington's disease gene (HD) and its rat homologue in brain and selected nonneural tissues. The HD transcript was expressed throughout the brain in both rat and human, especially in the neurons of the dentate gyrus and pyramidal neurons of the hippocampal formation, cerebellar granule cell layer, cerebellar Purkinje cells and pontine nuclei. Other brain areas expressed lower levels of the HD transcript without pronounced regional differences. Neuronal expression predominated over glial expression in all regions. HD mRNA was also expressed in colon, liver, pancreas and testes. The regional specificity of neuropathology in HD, which is most prominent in the basal ganglia, thus cannot be accounted for by the pattern of expression of HD.
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