Nature Genetics
5, 17 - 21 (1993)
doi:10.1038/ng0993-17
High resolution visual mapping of stretched DNA by fluorescent hybridizationIrma Parra1
& Bradford Windle1
1The Cancer Therapy and Research Center, 8122 Datapoint Drive, San Antonio, Texas 78229, USA Correspondence should be addressed to B.W. We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi−colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a >200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.
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