Gill Bejerano and colleagues (PLoS Genet. 9, e1003728, 2013) have generated a map of active enhancers in the region of the developing mouse brain that gives rise to the neocortex. The authors dissected the dorsal cerebral wall at embryonic day (E) 14.5 and performed chromatin immunoprecipitation and sequencing (ChIP-seq) with an antibody against p300, a marker of active enhancers. They identified 6,629 sites enriched for p300 binding, 95% of which show evolutionary conservation in humans and 20% of which overlap with a previously published map of p300 binding sites from E11.5 mouse forebrain (Cell 152, 895–908, 2013). They subsequently tested ten of the newly discovered candidate enhancer elements in transgenic reporter assays and found that eight of them could drive expression reproducibly in the developing neocortex. The predicted enhancers are enriched near genes known to be important for brain development, including several key transcription factors and signaling molecules. They are also enriched for transcription factor binding motifs for known regulators of neocortex development. The authors also identified several genes showing a very high density of p300 peaks. This new enhancer map provides a framework for studying the regulatory networks driving neocortical development and evolution.