Increasing crop yield is one of the most important goals of plant science research. Grain size is a major determinant of grain yield in cereals and is a target trait for both domestication and artificial breeding1. We showed that the quantitative trait locus (QTL) GS5 in rice controls grain size by regulating grain width, filling and weight. GS5 encodes a putative serine carboxypeptidase and functions as a positive regulator of grain size, such that higher expression of GS5 is correlated with larger grain size. Sequencing of the promoter region in 51 rice accessions from a wide geographic range identified three haplotypes that seem to be associated with grain width. The results suggest that natural variation in GS5 contributes to grain size diversity in rice and may be useful in improving yield in rice and, potentially, other crops2.
At a glance
- Genetic and molecular bases of rice yield. Annu. Rev. Plant Biol. 61, 421–442 (2010). &
- Strategies for developing green super rice. Proc. Natl. Acad. Sci. USA 104, 16402–16409 (2007).
- Control of tillering in rice. Nature 422, 618–621 (2003). et al.
- The OsTB1 gene negatively regulates lateral branching in rice. Plant J. 33, 513–520 (2003). et al.
- Cytokinin oxidase regulates rice grain production. Science 309, 741–745 (2005). et al.
- Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat. Genet. 40, 761–767 (2008). et al.
- Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. Plant J. 58, 592–605 (2009). et al.
- Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat. Genet. 42, 541–544 (2010). et al.
- OsSPL14 promotes panicle branching and higher grain productivity in rice. Nat. Genet. 42, 545–549 (2010). et al.
- DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol. 153, 1747–1758 (2010). et al.
- A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in Rice. Mol. Plant 4, 319–330 (2011). et al.
- GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor. Appl. Genet. 112, 1164–1171 (2006). et al.
- Evolutionary history of GS3, a gene conferring grain size in rice. Genetics 182, 1323–1334 (2009). et al.
- Linking differential domain functions of the GS3 protein to natural variation of grain size in rice. Proc. Natl. Acad. Sci. USA 107, 19579–19584 (2010). et al.
- QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat. Genet. 39, 623–630 (2007). , , , &
- Deletion in a gene associated with grain size increased yields during rice domestication. Nat. Genet. 40, 1023–1028 (2008). et al.
- Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight. Cell Res. 18, 1199–1209 (2008). et al.
- Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nat. Genet. 40, 1370–1374 (2008). et al.
- Brassinosteroids regulate grain filling in rice. Plant Cell 20, 2130–2145 (2008). et al.
- Analyzing quantitative trait loci for yield using a vegetatively replicated F2 population from a cross between the parents of an elite rice hybrid. Theor. Appl. Genet. 101, 248–254 (2000). et al.
- Genetic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid. Theor. Appl. Genet. 101, 823–829 (2000). et al.
- Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice. Theor. Appl. Genet. 105, 248–257 (2002). et al.
- Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Proc. Natl. Acad. Sci. USA 94, 9226–9231 (1997). et al.
- Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science 301, 376–379 (2003). et al.
- Generation and analysis of an artificial gene dosage series in tomato to study the mechanisms by which the cloned quantitative trait locus fw2.2 controls fruit size. Plant Physiol. 132, 292–299 (2003). , &
- Generation of flanking sequence-tag database for activation-tagging lines in japonica rice. Plant J. 45, 123–132 (2006). et al.
- T-DNA insertional mutagenesis for functional genomics in rice. Plant J. 22, 561–570 (2000). et al.
- The plant cell cycle-15 years on. New Phytol. 174, 261–278 (2007).
- Green light for the cell cycle. EMBO J. 24, 657–662 (2005).
- Cell cycle regulation in plant development. Annu. Rev. Genet. 40, 77–105 (2006). &
- The ins and outs of the plant cell cycle. Nat. Rev. Mol. Cell Biol. 8, 655–665 (2007). , &
- The plant cell cycle. Annu. Rev. Plant Biol. 54, 235–264 (2003). &
- fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289, 85–88 (2000). et al.
- Natural alleles at a tomato fruit size quantitative trait locus differ by heterochronic regulatory mutations. Proc. Natl. Acad. Sci. USA 99, 13606–13611 (2002). , &
- Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J. 6, 271–282 (1994). , , &
- Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25, 402–408 (2001). &
- Supplementary Text and Figures (840K)
Supplementary Tables 1–10 and Supplementary Figures 1–5