Brief Communication abstract
Nature Genetics 41, 166 - 167 (2009)
Published online: 25 January 2009 | doi:10.1038/ng.308
System-wide molecular evidence for phenotypic buffering in Arabidopsis
Jingyuan Fu1,2,9, Joost J B Keurentjes3,4,5,9, Harro Bouwmeester4,5,6, Twan America5,6, Francel W A Verstappen4,5,6, Jane L Ward7, Michael H Beale7, Ric C H de Vos5,6, Martijn Dijkstra1, Richard A Scheltema1, Frank Johannes1, Maarten Koornneef3,8, Dick Vreugdenhil4, Rainer Breitling1 & Ritsert C Jansen1,2
We profiled 162 lines of Arabidopsis for variation in transcript, protein and metabolite abundance using mRNA microarrays, two-dimensional polyacrylamide gel electrophoresis, gas chromatography time-of-flight mass spectrometry, liquid chromatography quadrupole time-of-flight mass spectrometry, and proton nuclear magnetic resonance. We added all publicly available phenotypic data from the same lines and mapped quantitative trait loci (QTL) for 40,580 molecular and 139 phenotypic traits. We found six QTL hot spots with major, system-wide effects, suggesting there are six breakpoints in a system otherwise buffered against many of the 500,000 SNPs.
- Groningen Bioinformatics Centre, University of Groningen, 9751NN Haren, The Netherlands.
- Department of Genetics, University Medical Centre Groningen, University of Groningen, 9700RB Groningen, The Netherlands.
- Laboratory of Genetics, Wageningen University, 6703BD Wageningen, The Netherlands.
- Laboratory of Plant Physiology, Wageningen University, 6703BD Wageningen, The Netherlands.
- Centre for Biosystems Genomics, Wageningen, 6708PB Wageningen, The Netherlands.
- Plant Research International, Wageningen, 6708PB Wageningen, The Netherlands.
- Rothamsted Research, National Centre for Plant and Microbial Metabolomics, AL5 2JQ Harpenden, Herts, UK.
- Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
- These authors contributed equally to this work.
Correspondence to: Ritsert C Jansen1,2 e-mail: r.c.jansen@rug.nl
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