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Nature Genetics 40, 1300–1306 (1 November 2008) | doi:10.1038/ng.235

Structure and function of a transcriptional network activated by the MAPK Hog1

Andrew P Capaldi , Tommy Kaplan , Ying Liu , Naomi Habib , Aviv Regev , Nir Friedman & Erin K O'Shea

Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.