Supplementary information : Ciliary proteins link basal body polarization to planar cell polarity regulation : Nature Genetics

From the following article

Ciliary proteins link basal body polarization to planar cell polarity regulation

Chonnettia Jones, Venus C Roper, Isabelle Foucher, Dong Qian, Boglarka Banizs, Christine Petit, Bradley K Yoder & Ping Chen

Nature Genetics 40, 69 - 77 (2008) Published online: 9 December 2007

doi:10.1038/ng.2007.54

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Supplementary Figures 1–5

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Supplementary Video 1

Three-dimensional images of cellular microtubules in a wild-type cochlea. Images of the cellular microtubules from three outer hair cells in a wild-type cochlea were acquired using the Zeiss LSM510 confocal microscope, as described in the text (Fig. 6). Serial 0.5m optical sections were acquired from the apical-most cell surface moving basally through the organ of corti and projected into three dimensions using NIH ImageJ software. This video complements Figure 6 and provides a more complete view of the apical microtubules.

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Supplementary Video 2

Three-dimensional images of cellular microtubules in an IFT88^CKO/CKO mutant cochlea. Images of the cellular microtubules from three outer hair cells in an IFT88^CKO/CKO mutant cochlea were acquired using the Zeiss LSM510 confocal microscope, as described in the text (Fig. 6). Serial 0.5m optical sections were acquired from the apical-most cell surface moving basally through the organ of corti and projected into three dimensions using NIH ImageJ software. This video complements Figure 6 and provides a more complete view of the apical microtubules.

Supplementary Video 2 - Download Movie (92KB)

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