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Letter

Nature Genetics 40, 113–117 (1 January 2008) | doi:10.1038/ng.2007.49

Identification and characterization of high-flux-control genes of yeast through competition analyses in continuous cultures

Daniela Delneri , David C Hoyle , Konstantinos Gkargkas , Emma J M Cross , Bharat Rash , Leo Zeef , Hui-Sun Leong , Hazel M Davey , Andrew Hayes , Douglas B Kell , Gareth W Griffith & Stephen G Oliver

Using competition experiments in continuous cultures grown in different nutrient environments (glucose limited, ammonium limited, phosphate limited and white grape juice), we identified genes that show haploinsufficiency phenotypes (reduced growth rate when hemizygous) or haploproficiency phenotypes (increased growth rate when hemizygous). Haploproficient genes (815, 1,194, 733 and 654 in glucose-limited, ammonium-limited, phosphate-limited and white grape juice environments, respectively) frequently show that phenotype in a specific environmental context.