Nature Genetics
4, 373 - 380 (1993)
doi:10.1038/ng0893-373
Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA libraryMark D. Adams1, M. Bento Soares2, Anthony R. Kerlavage1, Chris Fields1
& J. Craig Venter1
1Receptor Biochemistry and Molecular Biology Section, NINDS/NIH, 9000 Rockville Pike, Bethesda, Maryland 20892, USA and The Institute for Genomic Research, 932 Clopper Road, Gaithersburg, Maryland 20878, USA
2Department of Psychiatry, College of Physicians and Surgeons of Columbia University and New York State Psychiatric Institute, 722 W. 168th St., New York, New York 10032, USA Correspondence should be addressed to J.C.V. at TIGR A human infant brain cDNA library, made specifically for production of expressed sequence tags (ESTs) was evaluated by partial sequencing of over 1,600 clones. Advantages of this library, constructed for EST sequencing, include the use of directional cloning, size selection, very low numbers of mitochondrial and ribosomal transcripts, short polyA tails, few non-recombinants and a broad representation of transcripts. 37% of the clones were identified, based on matches to over 320 different genes in the public databases. Of these, two proteins similar to the Alzheimer's disease amyloid precursor protein were identified.
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