Article abstract
Nature Genetics 39, 178 - 188 (2007)
Published online: 14 January 2007 | doi:10.1038/ng1938
The quantitative trait gene latexin influences the size of the hematopoietic stem cell population in mice
Ying Liang1, Michael Jansen2,3, Bruce Aronow2, Hartmut Geiger3 & Gary Van Zant1
Abstract
We mapped quantitative trait loci that accounted for the variation in hematopoietic stem cell (HSC) numbers between young adult C57BL/6 (B6) and DBA/2 (D2) mice. In reciprocal chromosome 3 congenic mice, introgressed D2 alleles increased HSC numbers owing to enhanced proliferation and self-renewal and reduced apoptosis, whereas B6 alleles had the opposite effects. Using oligonucleotide arrays, real-time PCR and protein blots, we identified latexin (Lxn), a gene whose differential transcription and expression was associated with the allelic differences. Expression was inversely correlated with the number of HSCs; therefore, ectopic expression of Lxn using a retroviral vector decreased stem cell population size. We identified clusters of SNPs upstream of the Lxn transcriptional start site, at least two of which are associated with potential binding sites for transcription factors regulating stem cells. Thus, promoter polymorphisms between the B6 and D2 alleles may affect Lxn gene expression and consequently influence the population size of hematopoietic stem cells.
- Departments of Internal Medicine and Physiology, University of Kentucky, Lexington, Kentucky 40536, USA.
- Department of Biomedical Informatics, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA.
- Division of Experimental Hematology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA.
Correspondence to: Gary Van Zant1 e-mail: gvzant1@uky.edu
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