Article abstract
Nature Genetics 39, 1369 - 1375 (2007)
Published online: 21 October 2007 | doi:10.1038/ng.2007.22
Genetic basis of proteome variation in yeast
Eric J Foss1,5, Dragan Radulovic2,5, Scott A Shaffer3, Douglas M Ruderfer4, Antonio Bedalov1, David R Goodlett3 & Leonid Kruglyak4
Abstract
Proper regulation of protein levels is essential for health, and abnormal levels of proteins are hallmarks of many diseases. A number of studies have recently shown that messenger RNA levels vary among individuals of a species and that genetic linkage analysis can be used to identify quantitative trait loci that influence these levels. By contrast, little is known about the genetic basis of variation in protein levels in genetically diverse populations, in large part because techniques for large-scale measurements of protein abundance lag far behind those for measuring transcript abundance. Here we describe a label-free, mass spectrometry–based approach to measuring protein levels in total unfractionated cellular proteins, and we apply this approach to elucidate the genetic basis of variation in protein abundance in a cross between two diverse strains of yeast. Loci that influenced protein abundance differed from those that influenced transcript levels, emphasizing the importance of direct analysis of the proteome.
- Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
- Florida Atlantic University, Boca Raton, Florida 33431, USA.
- University of Washington, Seattle, Washington 98195, USA.
- Lewis-Sigler Institute for Integrative Genomics and Department of Ecology and Evolutionary Biology, Princeton University, Princeton, New Jersey 08544, USA.
- These authors contributed equally to this work.
Correspondence to: Leonid Kruglyak4 e-mail: leonid@genomics.princeton.edu
Correspondence to: Dragan Radulovic2,5 e-mail: radulovi@fau.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Toward the phosphoproteomeNature Biotechnology Research News (01 Apr 2001)
Shotgun mass spec goes independentNature Methods News and Views (01 Oct 2004)
See all 5 matches for News And ViewsRESEARCH
Functional expression and germline atransmission of a human chromosome fragment in chimaeric miceNature Genetics Article (01 Jun 1997)
Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS)Nature Biotechnology Research (01 Jun 2001)
See all 47 matches for Research
