Article abstract

Nature Genetics 39, 1235 - 1244 (2007)
Published online: 16 September 2007 | doi:10.1038/ng2117

A high-resolution atlas of nucleosome occupancy in yeast

William Lee1,2, Desiree Tillo3, Nicolas Bray3, Randall H Morse4, Ronald W Davis1,2, Timothy R Hughes3,5,6 & Corey Nislow3,5,6

We present the first complete high-resolution map of nucleosome occupancy across the whole Saccharomyces cerevisiae genome, identifying over 70,000 positioned nucleosomes occupying 81% of the genome. On a genome-wide scale, the persistent nucleosome-depleted region identified previously in a subset of genes demarcates the transcription start site. Both nucleosome occupancy signatures and overall occupancy correlate with transcript abundance and transcription rate. In addition, functionally related genes can be clustered on the basis of the nucleosome occupancy patterns observed at their promoters. A quantitative model of nucleosome occupancy indicates that DNA structural features may account for much of the global nucleosome occupancy.

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  1. Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA.
  2. Stanford Genome Technology Center, 855 California Avenue, Palo Alto, California 94304, USA.
  3. Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
  4. Wadsworth Center, New York State Department of Health, Albany, New York 12201-2002, USA.
  5. Terrence Donnelly Centre for Cellular & Biomolecular Research, 160 College Street, Toronto, Ontario M5S 3E1, Canada.
  6. Banting and Best Department of Medical Research, 112 College St., Toronto, Ontario M5S 1L6, Canada.

Correspondence to: Corey Nislow3,5,6 e-mail: corey.nislow@utoronto.ca

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