Nature Genetics 38, 758 - 769 (2006)
Published online: 25 June 2006; | doi:10.1038/ng1827
Bidirectional expression of CUG and CAG expansion transcripts and intranuclear polyglutamine inclusions in spinocerebellar ataxia type 8Melinda L Moseley, Tao Zu, Yoshio Ikeda, Wangcai Gao, Anne K Mosemiller, Randy S Daughters, Gang Chen, Marcy R Weatherspoon, H Brent Clark, Timothy J Ebner, John W Day&
Laura P W Ranum Supplementary Fig. 1 (pdf 76K)
Immunohistochemistry of TATA binding protein. Supplementary Fig. 2 (pdf 100K)
Immunohistochemistry with polyglutamine-specific MW5 antibody. Supplementary Fig. 3 (pdf 28K)
Ataxin 8 (ATXN8) ORF and flanking sequence. Supplementary Fig. 4 (pdf 72K)
Genomic organization of SCA8 region and Klhl1 expression in BAC transgenic mice. Supplementary Table 1 (pdf 12K)
Primer sequences. Supplementary Video 1 (mov 2M)
SCA8 BAC-Exp2 mouse. A high-copy SCA8 BAC-expansion animal (BAC-Exp2 at 20 weeks) displays severe motor deficits including stiffness and posturing with hindlimbs fully extended and unable to relax. Supplementary Video 2 (mov 692K)
SCA8 BAC-Exp5 mouse. A single-copy SCA8 BAC-expansion animal (BAC-Exp5 at 19 months) displays milder motor deficits including abnormal gait, low stance and hindlimb dragging. Supplementary Video 3 (mov 1M)
SCA8 BAC-Control mouse. A single-copy SCA8 BAC-control animal (BAC-Ctl1 at 18 months) shows no motor phenotype.
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