Nature Genetics
37, 980 - 985 (2005)
Published online: 14 August 2005; | doi:10.1038/ng1622
Regulation of polarized extension and planar cell polarity in the cochlea by the vertebrate PCP pathwayJianbo Wang1, 5, Sharayne Mark2, 5, Xiaohui Zhang2, 5, Dong Qian2, 5, Seung-Jong Yoo2, Kristen Radde-Gallwitz2, Yanping Zhang3, Xi Lin3, Andres Collazo4, Anthony Wynshaw-Boris1
& Ping Chen2, 31
Department of Pediatrics and Medicine, University of California San Diego School of Medicine, La Jolla, California 92093, USA. 2
Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA. 3
Department of Otolaryngology, Emory University School of Medicine, Atlanta, Georgia 30322, USA. 4
Department of Cell and Molecular Biology, House Ear Institute, Los Angeles, California 90057, USA. 5
These authors contributed equally to this work.
Correspondence should be addressed to Anthony Wynshaw-Boris awynshawboris@ucsd.edu or Ping Chen pchen@cellbio.emory.edu The mammalian auditory sensory organ, the organ of Corti, consists of sensory hair cells with uniformly oriented stereocilia on the apical surfaces and has a distinct planar cell polarity (PCP) parallel to the sensory epithelium1,
2,
3. It is not certain how this polarity is achieved during differentiation4,
5. Here we show that the organ of Corti is formed from a thicker and shorter postmitotic primordium through unidirectional extension, characteristic of cellular intercalation known as convergent extension6. Mutations in the PCP pathway interfere with this extension, resulting a shorter and wider cochlea as well as misorientation of stereocilia. Furthermore, parallel to the homologous pathway in Drosophila melanogaster
7,
8, a mammalian PCP component Dishevelled2 shows PCP-dependent polarized subcellular localization across the organ of Corti. Taken together, these data suggest that there is a conserved molecular mechanism for PCP pathways in invertebrates and vertebrates and indicate that the mammalian PCP pathway might directly couple cellular intercalations to PCP establishment in the cochlea.
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