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Technical Report
Nature Genetics 37, 1281 - 1288 (2005)
Published online: 2 October 2005; | doi:10.1038/ng1650

Second-generation shRNA libraries covering the mouse and human genomes

Jose M Silva, Mamie Z Li, Ken Chang, Wei Ge, Michael C Golding, Richard J Rickles, Despina Siolas, Guang Hu, Patrick J Paddison, Michael R Schlabach, Nihar Sheth, Jeff Bradshaw, Julia Burchard, Amit Kulkarni, Guy Cavet, Ravi Sachidanandam, W Richard McCombie, Michele A Cleary, Stephen J Elledge & Gregory J Hannon

Supplementary Fig. 1 (pdf 20K)
Mapping of Dicer and Drosha cleavage sites.

Supplementary Fig. 2 (pdf 104K)
The complete insert sequences for pSM1 and pSM2 containing a luciferase shRNA are shown along with their most stable potential secondary structures as predicted by RNA fold.

Supplementary Fig. 3 (pdf 216K)
Stable suppression by pSM2.

Supplementary Table 1 (pdf 88K)
ShRNAs used in Figure 3.

Supplementary Table 2 (pdf 44K)
ShRNAs used in Figure 4.

Supplementary Table 3 (pdf 20K)
Oligonucleotides used in construction of the library vectors.


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Nature Genetics
ISSN: 1061-4036
EISSN: 1546-1718
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