Nature Genetics 37, 1281 - 1288 (2005)
Published online: 2 October 2005; | doi:10.1038/ng1650
Second-generation shRNA libraries covering the mouse and human genomesJose M Silva, Mamie Z Li, Ken Chang, Wei Ge, Michael C Golding, Richard J Rickles, Despina Siolas, Guang Hu, Patrick J Paddison, Michael R Schlabach, Nihar Sheth, Jeff Bradshaw, Julia Burchard, Amit Kulkarni, Guy Cavet, Ravi Sachidanandam, W Richard McCombie, Michele A Cleary, Stephen J Elledge
& Gregory J Hannon Supplementary Fig. 1 (pdf 20K) Mapping of Dicer and Drosha cleavage sites. Supplementary Fig. 2 (pdf 104K) The complete insert sequences for pSM1 and pSM2 containing a luciferase shRNA are shown along with their most stable potential secondary structures as predicted by RNA fold. Supplementary Fig. 3 (pdf 216K) Stable suppression by pSM2. Supplementary Table 1 (pdf 88K) ShRNAs used in Figure 3. Supplementary Table 2 (pdf 44K) ShRNAs used in Figure 4. Supplementary Table 3 (pdf 20K) Oligonucleotides used in construction of the library vectors.
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