Nature Genetics 37, 1187 - 1193 (2005)
Published online: 27 October 2005; | doi:10.1038/ng1668
Current issues in mouse genome engineeringStefan Glaser, Konstantinos Anastassiadis
& A Francis Stewart
Genomics, BioInnovationsZentrum, Dresden University of Technology, Am Tatzberg 47, 01307, Dresden, Germany.
Correspondence should be addressed to A Francis Stewart stewart@biotec.tu-dresden.de The mouse is the foremost vertebrate experimental model because its genome can be precisely and variously engineered. Now that the mouse genome has been sequenced and annotated, the task of mutating each gene is feasible, and an international cooperation is providing mutated embryonic stem cells and mice as readily available resources. Because these resources will change biomedical research, decisions about their nature will have far-reaching effects. It is therefore timely to consider topical issues for mouse genome engineering, such as the background genotype; homologous, site-specific and transpositional recombination; conditional mutagenesis; RNA-mediated interference; and functional genomics with embryonic stem cells.
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