Nature Genetics
36, 867 - 871 (2004)
Published online: 4 July 2004; | doi:10.1038/ng1388
Mutagenic Insertion and Chromosome Engineering Resource (MICER)David J Adams1, Patrick J Biggs1, Tony Cox1, Rob Davies1, Louise van der Weyden1, Jos Jonkers1, 2, James Smith1, Bob Plumb1, Ruth Taylor1, Ichiko Nishijima3, Yuejin Yu3, Jane Rogers1
& Allan Bradley11
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs, CB10 1SA, UK. 2
The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. 3
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
Correspondence should be addressed to Allan Bradley abradley@sanger.ac.ukEmbryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting vectors from two libraries. 5,925 of these vectors can be used directly to inactivate genes with an average targeting efficiency of 28%. Combinations of vectors from the two libraries can be used to disrupt both alleles of a gene or engineer larger genomic changes such as deletions, duplications, translocations or inversions. These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome.
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