Access

Letter

Nature Genetics 36, 707–713 (1 July 2004) | doi:10.1038/ng1385

Identification of TFB5, a new component of general transcription and DNA repair factor IIH

Jeffrey A Ranish , Steven Hahn , Yu Lu , Eugene C Yi , Xiao-jun Li , Jimmy Eng & Ruedi Aebersold

We previously described the use of quantitative proteomics to study macromolecular complexes. Applying the method to analyze a yeast RNA polymerase II preinitiation complex, we identified a new 8-kDa protein, encoded by the uncharacterized open reading frame YDR079c-a, as a potential new component of the preinitiation complex.