Nature Genetics
36, 299 - 303 (2004)
Published online: 15 February 2004; | doi:10.1038/ng1307
A tiling resolution DNA microarray with complete coverage of the human genomeAdrian S Ishkanian1, Chad A Malloff1, Spencer K Watson1, Ronald J deLeeuw1, Bryan Chi1, Bradley P Coe1, Antoine Snijders2, Donna G Albertson2, Daniel Pinkel2, Marco A Marra3, Victor Ling1, Calum MacAulay1
& Wan L Lam11
British Columbia Cancer Research Centre, 601 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada. 2
UCSF Comprehensive Cancer Center, 2340 Sutter Street, San Francisco, California 94115, USA. 3
Genome Sciences Centre, British Columbia Cancer Agency, 600 West 10th Avenue, Vancouver BC V5Z 4E6, Canada.
Correspondence should be addressed to Wan L Lam wanlam@bccrc.caWe constructed a tiling resolution array consisting of 32,433 overlapping BAC clones covering the entire human genome. This increases our ability to identify genetic alterations and their boundaries throughout the genome in a single comparative genomic hybridization (CGH) experiment. At this tiling resolution, we identified minute DNA alterations not previously reported. These alterations include microamplifications and deletions containing oncogenes, tumor-suppressor genes and new genes that may be associated with multiple tumor types. Our findings show the need to move beyond conventional marker-based genome comparison approaches, that rely on inference of continuity between interval markers. Our submegabase resolution tiling set for array CGH (SMRT array) allows comprehensive assessment of genomic integrity and thereby the identification of new genes associated with disease.
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