Free energy lights the path toward more effective RNAi
Jose M Silva, Ravi Sachidanandam
& Gregory J Hannon
Jose M. Silva, Ravi Sachidanandam and Gregory J. Hannon are at Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA. hannon@cshl.org
Silencing gene expression by RNA interference (RNAi) has become a daily activity for mammalian cell biologists. Those who often use this approach have come to appreciate its occasional frustrations and potential limitations. A pair of recent papers in Cell provides rules for designing short interfering RNAs (siRNAs) that are both validated at the practical level and understood mechanistically.
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