Access

Brief Communication

Nature Genetics 34, 263–264 (1 July 2003) | doi:10.1038/ng1173

Induction of an interferon response by RNAi vectors in mammalian cells

Alan J Bridge , Stephanie Pebernard , Annick Ducraux , Anne-Laure Nicoulaz & Richard Iggo

DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex.