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Journal home Advance online publication Current issue Archive Press releases Free Association (blog) Supplements Focuses Guide to authors Online submission For referees Free online issue Contact the journal Subscribe Advertising work@npg Reprints and permissions About this site For librarians   NPG Resources Nature Nature Biotechnology Nature Cell Biology Nature Medicine Nature Methods Nature Reviews Cancer Nature Reviews Genetics Nature Reviews Molecular Cell Biology news@nature.com Nature Conferences Nature Reports Stem Cells RNAi Gateway NPG Subject areas Biotechnology Cancer Chemistry Clinical Medicine Dentistry Development Drug Discovery Earth Sciences Evolution & Ecology Genetics Immunology Materials Science Medical Research Microbiology Molecular Cell Biology Neuroscience Pharmacology Physics Browse all publications Letter Nature Genetics  34, 85 - 90 (2003) Published online: 21 April 2003; | doi:10.1038/ng1151 There is an Erratum (June 2003) associated with this Letter. Treatment-specific changes in gene expression discriminate in vivo drug response in human leukemia cells Meyling H. Cheok1, 2, Wenjian Yang1, Ching-Hon Pui3, 4, James R. Downing5, Cheng Cheng6, Clayton W. Naeve7, Mary V. Relling1, 4 & William E. Evans1, 4 1  The Hematological Malignancies Program: Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, 332 N. Lauderdale St., Memphis, Tennessee 38105 USA 2  The University of Bonn, Germany 3  Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA 4  The University of Tennessee, Memphis, USA 5  Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA. 6  Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, Tennessee, USA. 7  The Hartwell Center for Bioinformatics and Biotechnology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA. Correspondence should be addressed to William E. Evans william.evans@stjude.orgTo elucidate the genomics of cellular responses to cancer treatment, we analyzed the expression of over 9,600 human genes in acute lymphoblastic leukemia cells before and after in vivo treatment with methotrexate and mercaptopurine given alone or in combination. Based on changes in gene expression, we identified 124 genes that accurately discriminated among the four treatments. Discriminating genes included those involved in apoptosis, mismatch repair, cell cycle control and stress response. Only 14% of genes that changed when these medications were given as single agents also changed when they were given together. These data indicate that lymphoid leukemia cells of different molecular subtypes share common pathways of genomic response to the same treatment, that changes in gene expression are treatment-specific and that gene expression can illuminate differences in cellular response to drug combinations versus single agents. MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated REVIEWS Treatment by design in leukemia, a meeting report, Philadelphia, Pennsylvania, December 2002 Leukemia Reviews (01 Dec 2003) RESEARCH A molecular signature of metastasis in primary solid tumors Nature Genetics Letters (01 Jan 2003) Flow cytometric follow-up of minimal residual disease in bone marrow gives prognostic information in children with acute lymphoblastic leukemia Leukemia Original Article (01 Jan 2003) BFM-oriented treatment for children with acute lymphoblastic leukemia without cranial irradiation and treatment reduction for standard risk patients: results of DCLSG protocol ALL-8 (1991–1996) Leukemia Original Article (27 May 2002) Benefit of intensified treatment for all children with acute lymphoblastic leukaemia: results from MRC UKALL XI and MRC ALL97 randomised trials Leukemia Original Article (24 Feb 2000)  See all 6 matches for Research  Top Abstract Previous | Next Table of contents Full text Download PDF Send to a friend Save this link Open Innovation Challenges Fast Growth of Transformed Soybean Shoots Deadline: Jul 15 2009 Reward: $10,000 USD A method for accelerating growth of soybean shoots is desired. Protect Enzyme from In Planta Degradation Deadline: Jul 15 2009 Reward: $20,000 USD A proposal for stable expression of an enzyme in corn seed is desired. More Challenges Powered by: nature jobs Research Scientist in Allergy Nestlé Research Center Lausanne, Switzerland Molecular & Human Genetics Indian Institute of Chemical Biology Kolkata India More science jobs Post a job for free Figures & Tables Supplementary info Export citation Search buyers guide:   ADVERTISEMENT

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