Nature Genetics
32, 509 - 514 (2002)
doi:10.1038/ng1034
Post-analysis follow-up and validation of microarray experimentsRodrigo F. Chuaqui, Robert F. Bonner, Carolyn J.M. Best, John W. Gillespie, Michael J. Flaig, Stephen M. Hewitt, John L. Phillips, David B. Krizman, Michael A. Tangrea, Mamoun Ahram, W. Marston Linehan, Vladimir Knezevic
& Michael R. Emmert-Buck
Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.
Correspondence should be addressed to Michael R. Emmert-Buck mbuck@helix.nih.govMeasurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.
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