 | Figure 1
Nature Genetics
32, 438 - 442 (2002)
Published online: 30 September 2002; | doi:10.1038/ng1005
Genome-wide DNA replication profile for Drosophila melanogaster: a link between transcription and replication timingDirk Schübeler, David Scalzo, Charles Kooperberg, Bas van Steensel, Jeffrey Delrow
& Mark Groudine | | | | Figure 1. Experimental strategy to measure the timing of DNA replication using spotted microarrays.
a, Cell-cycle profile of D. melanogaster cells after pulse-labeling with BrdU and staining of DNA with propidium iodide. Cells between the G1 and G2 peaks are in S phase. Two fractions, reflecting the early (e) and late (l) stages of S phase, were sorted by FACS. b, After purification from the sorted cells, we enriched DNA containing BrdU by immunoprecipitation with an antibody specific for BrdU, and then carried out PCR amplification and fluorescent labeling. c, Early and late fractions were hybridized simultaneously to a spotted microarray. A section of one of the array hybridizations is shown. Enrichment of a sequence in early or late fractions is indicated by green or red fluorescence, respectively.
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