Nature Genetics30, 255 - 256 (2002)
Published online: 19 February 2002; | doi:10.1038/ng847
A gene-driven approach to the identification of ENU mutants in the mouse
Emma L. Coghill1, 3, Alison Hugill1, 3, Nick Parkinson1, Claire Davison1, Peter Glenister1, Sian Clements1, Jackie Hunter2, Roger D. Cox1
& Steve D.M. Brown1
1
MRC Mammalian Genetics Unit and UK Mouse Genome Centre, Harwell OX11 ORD, UK.
2
GlaxoSmithKline, New Frontiers Science Park, Harlow CM19 5AW, UK.
The construction of parallel archives of DNA and sperm from mice mutagenized with ethylnitrosurea (ENU) represents a potentially powerful and rapid approach for identifying point mutations in any gene in the mouse genome. We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program.
