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An Flp indicator mouse expressing alkaline phosphatase from the ROSA26 locus

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Figure 1: Widespread embryonic PLAP expression from the targeted R26FRAPki locus following Flpe-mediated excisions.
Figure 2: Generalized PLAP expression in postnatal R26:FRAP R26:FLPe mice.

References

  1. Dymecki, S.M. Proc. Natl Acad. Sci. USA 93, 6191–6196 (1996).

    Article  CAS  Google Scholar 

  2. Dymecki, S. & Tomasiewicz, H. Dev. Biol. 201, 57–65 (1998).

    Article  CAS  Google Scholar 

  3. Rodriguez, C.I. et al. Nature Genet. 25, 139–140 (2000).

    Article  CAS  Google Scholar 

  4. Rodriguez, C.I. & Dymecki, S.M. Neuron 27, 475–486 (2000).

    Article  CAS  Google Scholar 

  5. Farley, F.W., Soriano, P., Steffen, L.S. & Dymecki, S.M. Genesis 28, 106–110 (2000).

    Article  CAS  Google Scholar 

  6. Buchholz, F., Angrand, P.-O. & Stewart, A.F. Nature Biotechnol. 16, 657–662 (1998).

    Article  CAS  Google Scholar 

  7. O'Gorman, S., Fox, D.T. & Wahl, G.M. Science 251, 1351–1355 (1991).

    Article  CAS  Google Scholar 

  8. Nakano, M. et al. Nucleic Acids Res. 29, E40 (2001).

    Article  CAS  Google Scholar 

  9. Friedrich, G. & Soriano, P. Genes Dev. 5, 1513–1523 (1991).

    Article  CAS  Google Scholar 

  10. Zambrowicz, B.P. et al. Proc. Natl Acad. Sci. USA 94, 3789–3794 (1997).

    Article  CAS  Google Scholar 

  11. Soriano, P. Nature Genet. 21, 70–71 (1999).

    Article  CAS  Google Scholar 

  12. Chen, C.M. et al. Dev. Biol. 214, 370–384 (1999).

    Article  CAS  Google Scholar 

  13. Fields-Berry, S.C., Halliday, A.L. & Cepko, C.L. Proc. Natl Acad. Sci. USA 89, 693–697 (1992).

    Article  CAS  Google Scholar 

  14. Lobe, C.G. et al. Dev. Biol. 208, 281–292 (1999).

    Article  CAS  Google Scholar 

  15. Halliday, A.L. & Cepko, C.L. Neuron 9, 15–26 (1992).

    Article  CAS  Google Scholar 

  16. Maxwell, I.H., Harrison, G.S., Wood, W.M. & Maxwell, F.A. Biotechniques 7, 276–280 (1989).

    CAS  PubMed  Google Scholar 

  17. Thyagarajan, B., Guimaraes, M.J., Groth, A.C. & Calos, M.P. Gene 244, 47–54 (2000).

    Article  CAS  Google Scholar 

  18. de Alboran, I.M. et al. Immunity 14, 45–55 (2001).

    Article  CAS  Google Scholar 

  19. Loonstra, A. et al. Proc. Natl Acad. Sci. USA 98, 9209–9214 (2001).

    Article  CAS  Google Scholar 

  20. Silver, D.P. & Livingston, D.M. Mol. Cell 8, 233–243 (2001).

    Article  CAS  Google Scholar 

  21. Schmidt, E.E., Taylor, D.S., Prigge, J.R., Barnett, S. & Capecchi, M.R. Proc. Natl Acad. Sci. USA 97, 13702–13707 (2000).

    Article  CAS  Google Scholar 

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Acknowledgements

We thank J. Flanagan for the pcDNA-PLAP plasmid that served as a source of the 3′ PLAP coding sequence. We also thank C. Rodriguez, K. Chapman, P. Corrin and P. Mueting-Nelson for their excellent technical support. Work in S.D.'s laboratory was supported in part by the Rita Allen Foundation. Work in P.S.'s laboratory was supported by grants from the National Institutes of Health (HD 24875 and HD 25326).

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Awatramani, R., Soriano, P., Mai, J. et al. An Flp indicator mouse expressing alkaline phosphatase from the ROSA26 locus. Nat Genet 29, 257–259 (2001). https://doi.org/10.1038/ng1101-257

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