Nature Genetics
29, 263 - 264 (2001)
Published online: 30 October 2001; | doi:10.1038/ng754
Assembly of microarrays for genome-wide measurement of DNA copy numberAntoine M. Snijders1, 2, Norma Nowak4, Richard Segraves1, Stephanie Blackwood1, 2, Nils Brown1, Jeffrey Conroy4, Greg Hamilton1, Anna Katherine Hindle1, 2, Bing Huey1, Karen Kimura1, Sindy Law1, 2, Ken Myambo1, Joel Palmer1, 2, Bauke Ylstra1, 2, Jingzhu Pearl Yue1, Joe W. Gray1, 3, Ajay N. Jain1, 2, 3, Daniel Pinkel1, 3
& Donna G. Albertson1, 2, 31
Comprehensive Cancer Center, University of California San Francisco, San Francisco, California 94143. 2
Cancer Research Institute, University of California San Francisco, San Francisco, California 94143. 3
Department of Laboratory Medicine, University of California San Francisco, San Francisco, California 94143. 4
Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York 14263.
Correspondence should be addressed to Donna G. Albertson albertson@cc.ucsf.eduWe have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05−0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.
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