 | Figure 3
Nature Genetics
28, 276 - 280 (2001)
doi:10.1038/90123
Tbx5 associates with Nkx2-5 and synergistically promotes cardiomyocyte differentiationYukio Hiroi, Sumiyo Kudoh, Koshiro Monzen, Yuichi Ikeda, Yoshio Yazaki, Ryozo Nagai
& Issei Komuro | | | | Figure 3. Binding of Tbx5 and Nkx2-5 to the Nppa promoter.
The DNA fragment of the Nppa promoter was labeled and incubated with Tbx5 and/or Nkx2-5. a, Tbx5 binds to the fragment (bp -254 to -227) in a dose-dependent manner. The fragment of bp -254 to -236, but not the fragment of bp -244 to -227, competes with this binding. b, Competition of the binding of Tbx5 to the labeled fragment (bp -254 to -236). In bold, the Nkx2-5 binding sequence and a 'half-site' of the palindromic T (brachyury)-binding sequence (Tbx5 site); small upper-case letters, mutated nucleotides. c, Nkx2-5, as well as Tbx5, binds to the fragment of bp -254 to -236. Incubation of both Tbx5 and Nkx2-5 with this DNA fragment produces a larger shifted band in addition to the Tbx5− and Nkx2-5−DNA complexes (complex).
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