 | Figure 1
Nature Genetics
28, 276 - 280 (2001)
doi:10.1038/90123
Tbx5 associates with Nkx2-5 and synergistically promotes cardiomyocyte differentiationYukio Hiroi, Sumiyo Kudoh, Koshiro Monzen, Yuichi Ikeda, Yoshio Yazaki, Ryozo Nagai
& Issei Komuro | | | | Figure 1. Association of Tbx5 and Nkx2-5.
a,b, Cell lysates containing FLAG-tagged Tbx5 and/or HA-tagged Nkx2-5 expression plasmids were incubated with anti-HA or anti-FLAG monoclonal antibody, then the immune complex was precipitated with anti-mouse IgG agarose beads and separated by SDS−PAGE. Immunoblotting with anti-FLAG antibody (a) or anti-HA monoclonal antibody (b) shows that Tbx5 and Nkx2-5 are associated in mammalian cells. c−e, The association of GST−Nkx2-5 fusion proteins and in vitro-translated Tbx5 labeled with [35S]methionine. c, Nkx2-5 deletion mutants and full-length Tbx5, showing the homeodomain of Nkx2-5 is necessary and sufficient for this association. Left, Nkx2-5 mutants. WT, Full-length protein;  N, deletion of N-terminal domain outside homeodomain; C, deletion of C-terminal domain outside homeodomain; HD, homeodomain; N, N-terminal domain. d, Tbx5 deletion mutants and full-length Nkx2-5, showing the N-terminal domain of Tbx5 outside of the T-box is necessary for this interaction. Numbers at left, amino acids of Tbx5 mutants. e, Deletion mutants of the C-terminal domain and T-box of Tbx5, and a full-length Nkx2-5, showing the N-terminal part of T-box is necessary for this interaction. Numbers at left, amino acids of Tbx5 mutants.
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