Abstract
Loss of heterozygosity (LOH) of markers on human chromosome 7q31 is frequently encountered in a variety of human neoplasias, indicating the presence of a tumor-suppressor gene (TSG). By a combination of microcell-fusion and deletion-mapping studies, we previously established that this TSG resides within a critical region flanked by the genetic markers D7S522 and D7S677. Using a positional cloning strategy and aided by the availability of near-complete sequence of this genomic interval, we have identified a TSG within 7q31, named ST7 (for suppression of tumorigenicity 7; this same gene was recently reported in another context and called RAY1). ST7 is ubiquitously expressed in human tissues. Analysis of a series of cell lines derived from breast tumors and primary colon carcinomas revealed the presence of mutations in ST7. Introduction of the ST7 cDNA into the prostate-cancer–derived cell line PC3 had no effect on the in vitro proliferation of the cells, but abrogated their in vivo tumorigenicity. Our data indicate that ST7 is a TSG within chromosome 7q31 and may have an important role in the development of some types of human cancer.
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Acknowledgements
We thank the Washington University Genome Sequencing Center for generating the high-quality sequence that facilitated the identification of ST7; S. Hoogstraten-Miller for assistance in the animal studies; and B. Vogelstein, F. Collins, P. Meltzer, A. Baxevanis, J. Thomas and L. Everett for critical review of the manuscript.
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Zenklusen, J., Conti, C. & Green, E. Mutational and functional analyses reveal that ST7 is a highly conserved tumor-suppressor gene on human chromosome 7q31. Nat Genet 27, 392–398 (2001). https://doi.org/10.1038/86891
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DOI: https://doi.org/10.1038/86891
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