Journal home
Advance online publication
Current issue
Press releases
Free Association (blog)
Guide to authors
Online submissionOnline submission
For referees
Free online issue
Contact the journal
Reprints and permissions
About this site
For librarians
NPG Resources
Nature Biotechnology
Nature Cell Biology
Nature Medicine
Nature Methods
Nature Reviews Cancer
Nature Reviews Genetics
Nature Reviews Molecular Cell Biology
Nature Conferences
RNAi Gateway
NPG Subject areas
Clinical Medicine
Drug Discovery
Earth Sciences
Evolution & Ecology
Materials Science
Medical Research
Molecular Cell Biology
Browse all publications
Nature Genetics  27, 48 - 54 (2001)

Transcriptional regulation and function during the human cell cycle

Raymond J. Cho1, 6, 10, Mingxia Huang2, 10, Michael J. Campbell3, 7, 10, Helin Dong4, Lars Steinmetz1, Lisa Sapinoso8, Garret Hampton8, Stephen J. Elledge2, Ronald W. Davis1, 5 & David J. Lockhart4, 9

1  Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.

2  Department of Biochemistry and Molecular Biology, Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas, USA.

3  Molecular Applications Group, Palo Alto, California, USA.

4  Affymetrix, Inc., Santa Clara, California, USA.

5  Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.

6  Present address: InGenuity Systems, Inc., Alviso, California, USA.

7  Present address: Celera Genomics, Foster City, California, USA.

8  Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.

9  Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, California, USA.

10  These authors contributed equally to this work.

Correspondence should be addressed to Michael J. Campbell
We report here the transcriptional profiling of the cell cycle on a genome-wide scale in human fibroblasts. We identified approximately 700 genes that display transcriptional fluctuation with a periodicity consistent with that of the cell cycle. Systematic analysis of these genes revealed functional organization within groups of coregulated transcripts. A diverse set of cytoskeletal reorganization genes exhibit cell-cycle−dependent regulation, indicating that biological pathways are redirected for the execution of cell division. Many genes involved in cell motility and remodeling of the extracellular matrix are expressed predominantly in M phase, indicating a mechanism for balancing proliferative and invasive cellular behavior. Transcripts upregulated during S phase displayed extensive overlap with genes induced by DNA damage; cell-cycle−regulated transcripts may therefore constitute coherent programs used in response to external stimuli. Our data also provide clues to biological function for hundreds of previously uncharacterized human genes.

Previous | Next
Table of contents
Full textFull text
Download PDFDownload PDF
Send to a friendSend to a friend
Save this linkSave this link


Figures & Tables
Export citation

Search buyers guide:

Nature Genetics
ISSN: 1061-4036
EISSN: 1546-1718
Journal home | Advance online publication | Current issue | Archive | Press releases | Supplements | Focuses | For authors | Online submission | Permissions | For referees | Free online issue | About the journal | Contact the journal | Subscribe | Advertising | work@npg | naturereprints | About this site | For librarians
Nature Publishing Group, publisher of Nature, and other science journals and reference works©2001 Nature Publishing Group | Privacy policy