 | Figure 3
Nature Genetics
25, 357 - 361 (2000)
doi:10.1038/77153
Loss-of-function mutations in TYROBP (DAP12) result in a presenile dementia with bone cystsJuha Paloneva, Marjo Kestilä, Jun Wu, Antti Salminen, Tom Böhling, Vesa Ruotsalainen, Panu Hakola, Alexander B.H. Bakker, Joseph H. Phillips, Petra Pekkarinen, Lewis L. Lanier, Tuomo Timonen
& Leena Peltonen | | | | Figure 3. Northern-blot analysis of TYROBP in multiple human tissues.
a, Selected dots from a human multiple tissue mRNA expression array hybridized using radiolabelled human TYROBP cDNA as a probe. 1, peripheral blood leukocytes; 2, spleen; 3, fetal spleen; 4, thymus; 5, bone marrow; 6, placenta; 7, lung; 8, fetal lung; 9, liver; 10, jejunum; 11, erythroleukaemia cell line K562; 12, whole brain; 13, fetal brain; 14, frontal lobe; 15, parietal lobe; 16, cerebellum; 17, cortex; 18, corpus callosum; 19, nucleus caudatus; 20, putamen; 21, thalamus; 22, hippocampus; 23, spinal cord; 24, genomic DNA (500 ng). b, Northern-blot analysis of different human tissues using the same probe as in (a) on multiple-tissue northern blots. Each lane contains 2  g poly(A)+ RNA from human tissues. Abundant steady-state mRNA was observed in haematological cells and tissues such as peripheral blood leukocytes and spleen. TYROBP transcripts were also found in almost equal quantity in bone marrow, lymph nodes, placenta, lung and liver. A distinct, but lower intensity, steady-state mRNA signal was detected in different parts of the brain, especially in the basal ganglia (nucleus caudatus and putamen) which are affected at an early stage of PLOSL, and in the corpus callosum. Relatively strong northern-blot signals were also detected in the medulla and spinal cord. PBL, peripheral blood leukocytes.
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