 | Figure 2
Nature Genetics
25, 357 - 361 (2000)
doi:10.1038/77153
Loss-of-function mutations in TYROBP (DAP12) result in a presenile dementia with bone cystsJuha Paloneva, Marjo Kestilä, Jun Wu, Antti Salminen, Tom Böhling, Vesa Ruotsalainen, Panu Hakola, Alexander B.H. Bakker, Joseph H. Phillips, Petra Pekkarinen, Lewis L. Lanier, Tuomo Timonen
& Leena Peltonen | | | | Figure 2. Northern (a) and western (b) analysis of TYROBP and DAP10 transcripts and corresponding polypeptides of patients carrying PLOSLFin mutations.
a, We analysed lymphoblast poly(A)+ blot using radiolabelled human TYROBP (encompassing exons 1−5) and DAP10 (encompassing exons 1−4) cDNA as probes. b, TYROBP and DAP10 polypeptides were immunoprecipitated with anti-TYROBP monoclonal antibody or an affinity-purified polyclonal antiserum against DAP10, or with a control mouse IgG1 monoclonal antibody or control rabbit IgG (cIg), respectively. Polypeptides from the Finnish PLOSL patients and control individuals were immunoprecipitated from the lysates of PBMC (TYROBP) and lymphoblasts (DAP10). Immunoprecipitates were then analysed by western blot using anti-TYROBP and anti-DAP10 antibodies. PBL, control peripheral blood leukocytes; LAK, control interleukin-2 activated killer cells; NK-92, control NK cell line.
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