Nature Genetics
25, 144 - 146 (2000)
doi:10.1038/75985
Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogeneDonna G. Albertson1, 2, Bauke Ylstra1, Richard Segraves2, Colin Collins1, 2, Shanaz H. Dairkee3, David Kowbel1, Wen-Lin Kuo2, Joe W. Gray2
& Daniel Pinkel21
Cancer Research Institute, University of California, San Francisco, Box 0808, San Francisco, California, USA. 2
Cancer Genetics and Breast Oncology Programs, UCSF Cancer Center, University of California, San Francisco, Box 0808, San Francisco, California, USA. 3
Geraldine Brush Cancer Research Institute, California Pacific Medical Center, San Francisco, California, USA.
Correspondence should be addressed to Donna G. Albertson albertson@cc.ucsf.eduWe show here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization1,
2,
3,
4 (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima. Using this analytical capability, we resolved two regions of amplification within an approximately 2-Mb region of recurrent aberration at 20q13.2 in breast cancer. The putative oncogene ZNF217 (ref. 5) mapped to one peak, and CYP24 (encoding vitamin D 24 hydroxylase), whose overexpression is likely to lead to abrogation of growth control mediated by vitamin D (ref. 6), mapped to the other.
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