Nature Genetics
23, 405 - 412 (1999)
doi:10.1038/70508
TIN2, a new regulator of telomere length in human cellsSahn-ho Kim, Patrick Kaminker
& Judith Campisi
Department of Cell and Molecular Biology, Lawrence
Berkeley National Laboratory, Berkeley, California,
USA.
Correspondence should be addressed to Judith Campisi jcampisi@lbl.govTelomeres are DNA-protein structures that cap linear chromosomes and are
essential for maintaining genomic stability and cell phenotype. We identified
a novel human telomere-associated protein, TIN2, by interaction cloning using
the telomeric DNA-binding-protein TRF1 as a bait. TIN2 interacted with TRF1 in vitro and in cells, and co-localized with TRF1 in nuclei and metaphase
chromosomes. A mutant TIN2 that lacks amino-terminal sequences effects elongated
human telomeres in a telomerase-dependent manner. Our findings suggest that
TRF1 is insufficient for control of telomere length in human cells, and that
TIN2 is an essential mediator of TRF1 function.
|
