Nature Genetics
22, 384 - 387 (1999)
doi:10.1038/11962
Radiation hybrid map of the mouse genomeWilliam J. Van Etten1, Robert G. Steen1, Huy Nguyen1, Andrew B. Castle1, Donna K. Slonim1, Bing Ge2, Chad Nusbaum1, Greg D. Schuler3, Eric S. Lander1, 4
& Thomas J. Hudson1, 21
Whitehead Institute/MIT Center for Genome Research, Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA. 2
Montreal General Hospital Research Institute, McGill University, Montreal, H3G 1A4, Canada. 3
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA. 4
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Correspondence should be addressed to Eric S. Lander lander@genome.wi.mit.edu or Thomas J. Hudson thudson@genome.wi.mit.eduRadiation hybrid (RH) maps are a useful tool for genome analysis, providing a direct method for localizing genes and anchoring physical maps and genomic sequence along chromosomes. The construction of a comprehensive RH map for the human genome1 has resulted in gene maps reflecting the location of more than 30,000 human genes2,
3. Here we report the first comprehensive RH map of the mouse genome. The map contains 2,486 loci screened against an RH panel of 93 cell lines4. Most loci (93%) are simple sequence length polymorphisms (SSLPs) taken from the mouse genetic map, thereby providing direct integration between these two key maps. We performed RH mapping by a new and efficient approach in which we replaced traditional gel- or hybridization-based assays by a homogeneous 5´-nuclease assay5 involving a single common probe for all genetic markers. The map provides essentially complete connectivity and coverage across the genome, and good resolution for ordering loci, with 1 centiRay (cR) corresponding to an average of approximately 100 kb. The RH map, together with an accompanying World-Wide Web server, makes it possible for any investigator to rapidly localize sequences in the mouse genome. Together with the previously constructed genetic map6 and a YAC-based physical map reported in a companion paper7, the fundamental maps required for mouse genomics are now available.
|
