Nature Genetics
22, 27 - 36 (1999)
doi:10.1038/8737
A radiation hybrid map of the rat genome containing 5,255 markersTakeshi K. Watanabe1, 8, Marie-Therese Bihoreau2, 8, Linda C. McCarthy3, 7, 8, Susanna L. Kiguwa3, 8, Haretsugu Hishigaki1, Atsushi Tsuji1, Julie Browne2, Yuki Yamasaki1, Ayako Mizoguchi-Miyakita1, Keiko Oga1, Toshihide Ono1, Shiro Okuno1, Naohide Kanemoto1, Ei-ichi Takahashi1, Kazuhiro Tomita1, Hiromi Hayashi1, Masakazu Adachi1, Caleb Webber2, Marie Davis3, Susanne Kiel2, Catherine Knights3, Angela Smith3, Ricky Critcher3, Jonathan Miller2, Thiru Thangarajah2, Philip J.R. Day2, James R. Hudson Jr4, Yasuo Irie1, Toshihisa Takagi5, Yusuke Nakamura6, Peter N. Goodfellow3, G. Mark Lathrop2, Akira Tanigami1
& Michael R. James21
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co. Ltd, 463-10 Kagasuno, Kawauchi-cho, Tokushima, 771-0192, Japan. 2
Wellcome Trust Centre for Human Genetics, Windmill Road, Oxford, OX3 7BN, UK.
3
Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge, CB2 3EH, UK. 4
Research Genetics, 2130 S. Memorial Parkway, Huntsville, Alabama 35801, USA. 5
Laboratory of Genome Database, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108, Japan. 6
Laboratory of Molecular Medicine, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108, Japan. 7
Present address: Molecular Genetics, GlaxoWellcome Medicines Research Centre, Gunnels Wood Road, Stevenage SGN 2NY, UK. 8
These authors contributed equally to this work.
Correspondence should be addressed to Michael R. James michael.james@well.ox.ac.uk or Atsushi TsujiAkira Tanigami atanigam@otsuka.gr.jpA whole-genome radiation hybrid (RH) panel was used to construct a high-resolution
map of the rat genome based on microsatellite and gene markers. These include
3,019 new microsatellite markers described here for the first time and 1,714
microsatellite markers with known genetic locations, allowing comparison and
integration of maps from different sources. A robust RH framework map containing
1,030 positions ordered with odds of at least 1,000:1 has been defined as
a tool for mapping these markers, and for future RH mapping in the rat. More
than 500 genes which have been mapped in mouse and/or human were localized
with respect to the rat RH framework, allowing the construction of detailed
rat-mouse and rat-human comparative maps and illustrating the power of the
RH approach for comparative mapping.
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