Resequencing and mutational analysis using oligonucleotide microarrays
Joseph G. Hacia
Genetics and Molecular Biology Branch, National Human Genome Research Institute, Building 49/3A14, National Institutes of Health, Bethesda, Maryland 20892, USA. jhacia@nhgri.nih.gov
Oligonucleotide microarray (DNA chip)−based hybridization analysis
is a promising new technology which potentially allows rapid and cost−effective
screens for all possible mutations and sequence variations in genomic DNA.
Here, I review current strategies and uses for DNA chip−based resequencing
and mutational analysis, the underlying principles of experimental designs,
and future efforts to improve the sensitivity and specificity of chip−based
assays.
