 | Figure 1
Nature Genetics
20, 66 - 69 (1998)
doi:10.1038/1727
Trisomy 7-harbouring non-random duplication of the mutant MET
allele in hereditary papillary renal carcinomasZhengping Zhuang, Won-Sang Park, Svetlana Pack, Laura Schmidt, Alexander O. Vortmeyer, Evgenia Pak, Thu Pham, Robert J. Weil, Sonja Candidus, Irina A. Lubensky, W. Marston Linehan, Berton Zbar
& Gregor Weirich | | | | Figure 1. FISH analysis of lymphoblastoid cell line and tumour tissues from HPRC
patients.
a, Two colour FISH of metaphase chromosomes prepared from lymphoblastoid
cell cultures obtained from patient 2. Cosmid clone c182b3, which contains
the MET gene (rhodamine, red signal) and a centromeric  -satellite
probe (FITC, green signal) specific for chromosome 7 as a reference marker.
b, Interphase FISH on tumour touch preparation showing the three copies
of MET (rhodamine, red signal), using c182b3 combined with a centromeric -satellite
probe (FITC, green signal) specific for chromosome 17. c, Two colour
FISH of metaphase chromosomes from patient 2, as above, using a chromosome
7-specific painting probe, which shows the two germline copies. d,
Interphase FISH showing three copies of chromosome 7 in one of the tumours
using the chromosome 7-specific painting probe (rhodamine, red signal) and
the chromosome 7 -satellite probe (FITC, green signal).
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