Nature Genetics
2, 196 - 199 (1992)
doi:10.1038/ng1192-196
Expression of the X−inactivation−associated gene XIST during spermatogenesisEduardo C. Salido1, 2, 5, Pauline H. Yen1, 3, Thuluvancheri K. Mohandas3
& Larry J. Shapiro1, 3, 4, 5
1Howard Hughes Medical Institute, UCLA School of Medicine, Harbor-UCLA Medical Center, Torrance, California 90509, USA
2Department of Pathology, University of La Laguna School of Medicine, E-38071 Tenerife, Spain
3Department of Pediatrics, UCLA School of Medicine, Harbor-UCLA Medical Center, Torrance, California 90509, USA
4Department of Biological Chemistry, UCLA School of Medicine, Harbor-UCLA Medical Center, Torrance, California 90509, USA
5Present address: Department of Pediatrics, University of California School of Medicine, San Francisco, California 94143, USA Mammalian X−chromosome inactivation is thought to be controlled by the X inactivation centre (XIC, X−controlling element −Xce− in mice). A human gene, XIST and its mouse counterpart, Xist, which map to the XIC/Xce, are expressed exclusively from inactive X chromosomes, suggesting their involvement in the process of X−inactivation. We now report the presence of Xist/XIST transcripts in newborn and adult mouse testes, and in human testicular tissue with normal spermatogenesis, but not in the testes of patients who lack germ cells. Our results indicate that while the X chromosome in males is active in somatic cells, it undergoes inactivation during spermatogenesis.
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