Nature Genetics
19, 79 - 82 (1998)
doi:10.1038/ng0598-79
Skeletal muscle-specific expression of a utrophin transgene rescues utrophin-dystrophin deficient miceJill A. Rafael1, Jonathon M. Tinsley1, Allyson C. Potter1, Anne E. Deconinck1
& Kay E. Davies1, 2
1Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
2e-mail: kdavies@bioch.ox.ac.uk Duchenne muscular dystrophy (DMD) is a progressive muscle wasting disease usually resulting in death of patients by their early twenties1. In contrast, mice lacking dystrophin (Dmdmdx), appear physically normal despite their underlying muscle pathology2,3. Mice deficient for both dystrophin and the dystrophin-related protein, utrophin, (Dmdmdx;Utm-/- mice) die between 6 and 20 weeks of age suffering from severe muscle weakness with joint contractures, pronounced growth retardation and kyphosis, suggesting that dystrophin and utrophin play complementary roles4,5. The exact cause of death in these mice was not determined. Here we show that expression of a truncated utrophin transgene solely within the skeletal muscle of these mutants prevents premature death and the development of any clinical phenotype. In the absence of full-length dystrophin and utrophin, the presence of truncated utrophin also decreases muscle fibre regeneration, relocalizes the dystrophin protein complex to the sarcolemma and re-establishes a normal expression pattern of developmental muscle proteins. These data suggest that Dmdmdx;Utrn-/- mice succumb to a skeletal muscle defect and that their reduced lifespan is not due to cardiac or neurogenic components. The phenotypic rescue observed demonstrates that the Dmdmdx;Utm-/- mice are an ideal model for testing gene delivery protocols for the expression of utrophin or dystrophin in skeletal muscle.
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