Nature Genetics
18, 338 - 343 (1998)
doi:10.1038/ng0498-338
A microsatellite genetic linkage map for zebrafish (Danio rerio)Ela W. Knapik1, 4, 6, Alec Goodman1, Marc Ekker2, Mario Chevrette3, Jonathan Delgado1, Stephan Neuhauss1, Nobuyoshi Shimoda1, Wolfgang Driever1, Mark C. Fishman1, 7
& Howard J. Jacob1, 5
1Cardiovascular Research Center, Massachusetts General Hospital Charlestown, Massachusetts 02129, USA.
2Loeb Institute for Medical Research, Ottawa Civic Hospital, University of Ottawa, 725 Parkdale Avenue, Ottawa, Ontario, Canada, K1Y4E9.
3Department of Surgery, Urology Division, McGill University and Montreal General Hospital Research Institute, 1650 Avenue Cedar, Montreal, Quebec, Canada, H3G 1A4.
4GSF, Research Center for Environment and Health, Institute of Mammilian Genetics, Ingolstädter Landstrasse 1, 85758 Neuherberg, Germany.
5Medical College of Wisconsin, Department of Physiology, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA.
6knapik@gsf.de
7fishman@cvrc-taco.mgh.harvard.edu We have constructed a zebrafish genetic linkage map consisting of 705 simple sequence-length polymorphism markers (SSLPs). The map covers 2350 centimorgans (cM) of the zebrafish genome with an average resolution of 3.3 cM. It is a complete map in genetic mapping terms (there is one linkage group for each of the 25 chromosomes), and it has been confirmed by somatic-cell hybrids and centromere-mapping using half-tetrad analysis. The markers are highly polymorphic in the zebrafish strains used for genetic crosses and provide a means to compare genetic segregation of developmental mutations between laboratories. These markers will provide an initial infrastructure for the positional cloning of the nearly 600 zebrafish genes identified as crucial to vertebrate developmentfand will become the anchor for the physical map of the zebrafish genome.
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